{
"@context": "/terms/",
"@id": "/sequence-files/IGVFFI0107JODV/",
"@type": [
"SequenceFile",
"File",
"Item"
],
"accession": "IGVFFI0107JODV",
"assay_titles": [
"CRISPR FlowFISH screen"
],
"audit": {},
"award": {
"@id": "/awards/HG011972/",
"component": "functional characterization"
},
"content_md5sum": "d99e34a0650873981b21c904faafbb85",
"content_type": "reads",
"controlled_access": false,
"creation_timestamp": "2024-04-16T21:42:54.290602+00:00",
"externally_hosted": false,
"file_format": "fastq",
"file_set": {
"@id": "/auxiliary-sets/IGVFDS9229NPJH/",
"accession": "IGVFDS9229NPJH",
"file_set_type": "gRNA sequencing",
"samples": [
{
"@id": "/in-vitro-systems/IGVFSM4639DMNG/",
"accession": "IGVFSM4639DMNG",
"classifications": [
"cell line"
],
"modifications": [
{
"@id": "/crispr-modifications/b4bc725a-8eeb-4180-9789-bece3516c0c1/",
"modality": "interference",
"status": "released",
"summary": "CRISPRi Sp-dCas9-KOX1-KRAB"
}
],
"sample_terms": [
{
"@id": "/sample-terms/NTR_0000753/",
"status": "released",
"term_name": "TeloHAEC"
}
],
"status": "released",
"summary": "TeloHAEC cell line, female, Homo sapiens (sorting details: FACS bin C for 51-75% expression of NOS3) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library",
"taxa": "Homo sapiens"
}
],
"status": "released",
"summary": "gRNA sequencing for CRISPR interference FlowFISH screen integrating a guide (sgRNA) library targeting sequence variants, transcription start sites in NOS3"
},
"file_size": 6541865,
"gene_list_for": [],
"href": "/sequence-files/IGVFFI0107JODV/@@download/IGVFFI0107JODV.fastq.gz",
"illumina_read_type": "I2",
"input_file_for": [],
"integrated_in": [],
"lab": {
"@id": "/labs/jesse-engreitz/",
"title": "Jesse Engreitz, Stanford"
},
"loci_list_for": [],
"maximum_read_length": 8,
"md5sum": "e74cab1534baee1a69c3f43a8eed08af",
"mean_read_length": 8,
"minimum_read_length": 8,
"read_count": 791378,
"release_timestamp": "2024-06-22T00:26:39.536559+00:00",
"s3_uri": "s3://igvf-files/2024/04/16/9eddd32c-6153-405a-aeab-e66421044021/IGVFFI0107JODV.fastq.gz",
"schema_version": "14",
"seqspecs": [],
"sequencing_kit": "MiSeq Reagent Kit v2",
"sequencing_platform": {
"@id": "/platform-terms/EFO_0004205/",
"status": "released",
"term_name": "Illumina MiSeq"
},
"sequencing_run": 1,
"status": "released",
"submitted_by": {
"@id": "/users/3bd6f74c-8cf3-4c35-a94d-0c7c11654815/",
"title": "Chongyon Park"
},
"submitted_file_name": "/Desktop/NOS3/NOS3_CRISPRi_FlowFISH/231215S023-TeloHAEC-P219-Amp_NOS3-BioRep1-FFrep1-BinC-PCRrep1_S115_I2_001.fastq.gz",
"summary": "I2 reads from sequencing run 1",
"upload_status": "validated",
"uuid": "9eddd32c-6153-405a-aeab-e66421044021"
}