IGVFFI0106GQNO

released

validated

File Set

IGVFDS3139RYUI(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_MYC-Auxin0hrs-FF2-BinC-PCR2_Rep1_S33

md5sum

0a62aa5bf852a84b5b8b63af641d4eb2

Content MD5sum

7a1ac02fdda59d204329b425728ea142

File Size

40 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220819B015_MYC/fastq_trim/MYC-Auxin0hrs-FF2-BinC-PCR2-Rep1_S33_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

CGTTACCA:TGCG

Average Read Length

Read Count

2071458

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM7258NLNH

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin C for 35-45% expression of MYC) modified with CRISPRi Sp-dCas9-KOX1-KRAB, inactive AID system targeting RAD21, transfected with a guide library