Home

IGVFFI0092MDDO

released
validated
File Set
IGVFDS6538ISBQ(unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I2 reads from sequencing run 2
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep4-FFrep1-FACS_Input-PCRrep2_I2
md5sum
31512387981fc0c5653c8ae032108ad3
Content MD5sum
955914a6a73d318d73a2d582dc1c9939
File Size
3.0 MB
Submitted File Name
220426pPPIF_REPEAT-THP1-Gen8b-pPPIF-Amp_pPPIF-BioRep2-FFRep1-BinAll-PCRrep2_S124_I2_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
2
Illumina Read Type
I2
Index
GACAGTAA:TGAC
Average Read Length
4
Read Count
389073

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford