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IGVFFI0075VJGZ

released
validated
File Set
IGVFDS4750CFGI(CRISPR prime editing Variant-EFFECTS sorted on the expression of PPIF integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep2-BinD-PCRrep11_I1
md5sum
3dc5e71727d0a1dcd713a7b02ec6d260
Content MD5sum
5adeb1608e5dc80f3e139a9a64393143
File Size
1.4 MB
Submitted File Name
230807S022-THP1-P153-Amp_PPIF_promoter-BioRep2-FFrep2-BinD-PCRrep11_S287_I1_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Jesse Engreitz, Stanford

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
I1
Index
TGTAACTC:ACTG
Average Read Length
8
Read Count
162610

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library