Home

IGVFFI0072NENY

released
validated
File Set
IGVFDS4863BMGZ(scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci)
Summary
I1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jay-shendure:CRISPRa_singlecell_K562_VP64_sequence_exprs_10Xlane1_seqlane1_I1
External Resources
md5sum
f8bad46e72cb599fcae4ca59c7a2619a
Content MD5sum
6400dd5ad4181748b73e2b4bde2c0bca
File Size
812 MB
Submitted File Name
/net/shendure/vol10/www/content/members/CRISPRa_QTL_website/public/data/K562_data/sequencing_data/cellranger_mkfastq_output/outs/fastq_path/AAAHG2HHV/VP64_exprs_1/VP64_exprs_1_S1_L001_I1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAAHG2HHV
Lane
1
Sequencing Kit
NextSeq 1000/2000 P3 Reagent Kit
Illumina Read Type
I1
Average Read Length
8
Read Count
124911858

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

Files Derived From This File

3 items
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
3.3 KB
validated
csv
differential gene expression quantifications
differential gene expression quantifications
Jay Shendure, UW
6.9 KB
validated
tar
sparse gene count matrix
cell by gene in sparse gene count matrix
Jay Shendure, UW
538 MB
validated

Attribution

Award
HG011966
Principal Investigator(s)
Contact P.I.
Jay Shendure
Lab
Jay Shendure, UW