IGVFFI0052KUVQ

released

validated

File Set

IGVFDS2855OFEM(gRNA sequencing for mixed CRISPR FlowFISH screen integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)

Summary

reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:auxiliary_file_CRUDO_SSFA2-Auxin6hrs-FF1-BinE-PCR3-Rep1_S83

md5sum

6270d44f80503b24b07bbfc40d2587b7

Content MD5sum

1533307cee9079296b04d3dbbffeb1a4

File Size

23 MB

Submitted File Name

/oak/stanford/groups/engreitz/Projects/Cohesin/220308B014_CCND1_SSFA2_FAM3C/fastq_trim/SSFA2-Auxin6hrs-FF1-BinE-PCR3-Rep1_S83_R1_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina HiSeq 2500

Sequencing Run

Index

TCGCCTTG:GCCA

Average Read Length

Read Count

1030077

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9082URPW

InVitroSystem

HCT116

HCT116 cell line, male, Homo sapiens (sorting details: FACS bin E for 80-90% expression of SSFA2) treated with 1 μM 5-Phenyl-1H-indole-3-acetic acid for 6 hours, modified with AID system targeting RAD21, CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library