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IGVFFI0046WWBL

released
validated
File Set
IGVFDS2504PFZO(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
jesse-engreitz:220228_8merInsertion-THP1-Amp_PPIF_promoter-BioRep1-FFrep1-BinD-PCRrep1_R1
md5sum
c1b9fda289b316f01d3d0f8fa0290cbf
Content MD5sum
0640ed0ed404976a04ac5f763451cb27
File Size
36 MB
Submitted File Name
220228pPPIF-FFSample-2-D21-pPPIF-Amp-pPPIF-1-1-1-BinD_R1_001.fastq.gz

Sequencing Details

Sequencing Platform
Illumina NextSeq 550
Sequencing Run
1
Illumina Read Type
R1
Index
GAAGAAGT:TGAC
Average Read Length
150
Read Count
1695931

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
THP-1 cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Attribution

Award
HG011972
Principal Investigator(s)
Contact P.I.
Jesse Engreitz
Lab
Jesse Engreitz, Stanford