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IGVFFI0029ZRXZ

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS6015JDKU(cell hashing barcode sequencing for CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW289C_1_R2
md5sum
cb0b5f1281614ac8c8055a7902d89e93
Content MD5sum
fd8dd1ec7ee77e6761ad31de3873fb6e
File Size
678 MB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-11-03-McDermott-NextSeq.10X.run2.10X/2023-11-03/outs/fastq_path/AAF3JCVM5//LW289C_S8_L001_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles
Attribution
Gary Hon, UT

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAF3JCVM5
Lane
1
Illumina Read Type
R2
Average Read Length
90
Read Count
16886184

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW289) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
589 MB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
429 MB
validated