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IGVFFI0029HYME

preview
validated
This object is in preview status. Metadata is subject to change until this object is released.
File Set
IGVFDS3495FUIW(CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
gary-hon:LW289_2_R2
md5sum
e77c55cc2ff2eac26c87ce5eba63057f
Content MD5sum
6bf0d9acbf589b64e193d9117c432bb9
File Size
6.9 GB
Submitted File Name
/project/GCRB/Hon_lab/shared/data/sequencing_data/2023/2023-11-03-McDermott-NextSeq.10X.run1/2023-11-03/outs/fastq_path/AAC7TGLHV//LW289_S4_L002_R2_001.fastq.gz
Checkfiles Version
Legacy version of checkfiles

Sequencing Details

Sequencing Platform
Illumina NextSeq 2000
Sequencing Run
1
Flowcell ID
AAC7TGLHV
Lane
2
Illumina Read Type
R2
Average Read Length
92
Read Count
122635682

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW289), male, Homo sapiens modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Files Derived From This File

2 items
pkl
raw feature barcode matrix
cell by CRISPR guide capture in raw feature barcode matrix
Gary Hon, UT
589 MB
validated
hdf5
filtered feature barcode matrix
cell by UMI count in filtered feature barcode matrix
Gary Hon, UT
429 MB
validated

Attribution

Award
HG011996
Principal Investigator(s)
Contact P.I.
Gary Hon
Lab
Gary Hon, UT