IGVFFI0026VHFY

preview

validated

This object is in preview status. Metadata is subject to change until this object is released.

File Set

IGVFDS0414OFOP(CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus)

Summary

I2 reads from sequencing run 1

File Format

fastq

Content Type

reads

Aliases

jesse-engreitz:Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-BinD-PCRRep6_I2

md5sum

efa8d64317b398da92d56dea539af1f7

Content MD5sum

02fed8b1f8aef7e503c4fe32cf83d3e4

File Size

2.0 MB

Submitted File Name

Jurkat_Stim-IL2RA_prom-BioRep1-FFRep1-PCRRep6-BinD_S124_I2_001.fastq.gz

Sequencing Details

Sequencing Platform

Illumina NovaSeq X

Sequencing Run

Illumina Read Type

Index

GACAGTAA:GGCA

Average Read Length

Read Count

260782

Samples

1 item

Accession

Type

Sample Terms

Summary

IGVFSM9103RFFD

InVitroSystem

Jurkat

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library