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IGVFFI0025FVRA

released
validated
File Set
IGVFDS8056EBUX(CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide)
Summary
R2 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
richard-sherwood:B4.2_18loci_Rep4_bulk_read2
md5sum
178ddddc16b3f6a15e984333cad479e0
Content MD5sum
dbf417db263fe4918218f36660ebd23c
File Size
7.1 MB
Submitted File Name
/net/bgm/sherwood/Tian_demult_data/nextseq_051523_Tian/fixed/B4.2_18loci_Rep4_bulk_R2.fastq.gz
Checkfiles Version
v17

Sequencing Details

Sequencing Platform
Element Biosciences AVITI
Sequencing Run
1
Illumina Read Type
R2
Average Read Length
60
Read Count
210871

Samples

1 item
Accession
Type
Sample Terms
Summary
InVitroSystem
HepG2 cell line, male, Homo sapiens modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
csv
guide quantifications
guide quantifications
Richard Sherwood, Brigham and Women's Hospital
336 KB
validated

Attribution

Award
HG012010
Principal Investigator(s)
Contact P.I.
Luca Pinello
Lab
Richard Sherwood, Brigham and Women's Hospital