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IGVFFI0020UHMA

released
validated
File Set
IGVFDS3760HQCL(CRISPR knockout RNA-seq integrating a guide (sgRNA) library targeting transcription start sites in ZNF217)
Summary
R1 reads from sequencing run 1
File Format
fastq
Content Type
reads
Aliases
charles-gersbach:cd8-tcells-rna-seq-cas-ko-znf217-rep1-fastq-R1
md5sum
0414d2b920825f64bcea73ca266fafc0
Content MD5sum
19b52eb48613578d15ba38c20feb3fad
File Size
2.3 GB
Submitted File Name
/data/reddylab/Alex/IGVF/data_submissions/raw_data/AIM_2_T_cell_project_1/pSM73-ZNF217-1_R1_001.fastq.gz
Submitter Comment
Libraries prepared and sequenced by Genewiz. Unknown sequencing platform (best guess annotated)

Sequencing Details

Sequencing Platform
Illumina NovaSeq 6000
Sequencing Run
1
Lane
1
Illumina Read Type
R1
Average Read Length
150
Read Count
30025193

Samples

1 item
Accession
Type
Sample Terms
Summary
PrimaryCell
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)

Files Derived From This File

1 item
Accession
File Format
Content Type
Summary
Lab
File Size
Upload Status
bam
transcriptome alignments
GRCh38 filtered transcriptome alignments
Charles Gersbach, Duke
2.2 GB
validated

Attribution

Award
HG012053
Principal Investigator(s)
Contact P.I.
Charles Gersbach
Lab
Charles Gersbach, Duke