CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of CCR7
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of CCR7
released
AuxiliarySet
IGVFDS7251RDFD
gRNA sequencing
Charles Gersbach, Duke
Summary
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
released
MeasurementSet
IGVFDS6373KKOW
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
Charles Gersbach, Duke
Sample Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)