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samples.@id
/primary-cells/IGVFSM7658WDNM/
3 items
  • MeasurementSet
    IGVFDS7661MIEF
    CRISPR interference FACS screen sorted on the expression of CD2 integrating a guide (sgRNA) library targeting transcription start sites in CD2
    Charles Gersbach, Duke
    Sample Summary
    Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
    released
  • AuxiliarySet
    IGVFDS8866USWP
    gRNA sequencing
    Charles Gersbach, Duke
    Summary
    gRNA sequencing
    revoked
  • principal
    AnalysisSet
    IGVFDS5157TBYH
    CRISPR interference FACS screen targeting CD2: alignments, fold change over control, guide quantifications
    Charles Gersbach, Duke
    Samples
    Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRi Sa-dCas9-KOX1-KRAB, transfected with a guide library, sorted on expression of CD2
    released