IGVF

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File Set Type

experimental data
1
gRNA sequencing
1
intermediate analysis
1
principal analysis
1

/primary-cells/IGVFSM7226CVDS/

4 items

AuxiliarySet
IGVFDS4215TFWE
gRNA sequencing
Charles Gersbach, Duke
Summary
gRNA sequencing for CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
released
MeasurementSet
IGVFDS7858OVUD
CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
Charles Gersbach, Duke
Sample Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
released
principal
AnalysisSet
IGVFDS5106XTZH
CRISPR knockout FACS screen targeting IL7R: fold change over control, guide quantifications
Charles Gersbach, Duke
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL7R
released
intermediate
AnalysisSet
IGVFDS5551EATG
CRISPR knockout FACS screen targeting IL7R: alignments
Charles Gersbach, Duke
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL7R
released