Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRa Sa-dCas9-VP64, transfected with a guide library, sorted on expression of CCR7
released
MeasurementSet
IGVFDS5281AOTI
low FACS signal CRISPR activation FACS screen sorted on the expression of CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes
Charles Gersbach, Duke
Sample Summary
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)