Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRa Sa-dCas9-VP64, transfected with a guide library, sorted on expression of CCR7
released
AuxiliarySet
IGVFDS6878SUMX
gRNA sequencing
Charles Gersbach, Duke
Summary
gRNA sequencing
revoked
MeasurementSet
IGVFDS6262JHMS
low FACS signal CRISPR activation FACS screen sorted on the expression of CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes
Charles Gersbach, Duke
Sample Summary
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)