low FACS signal CRISPR interference FACS screen sorted on the expression of B2M integrating a guide (sgRNA) library targeting transcription start sites in B2M
Charles Gersbach, Duke
Sample Summary
Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)
Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRi Sa-dCas9-KOX1-KRAB, transfected with a guide library, sorted on expression of B2M