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3 items
  • AuxiliarySet
    IGVFDS3945FAEL
    gRNA sequencing
    Charles Gersbach, Duke
    Summary
    gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
    released
  • MeasurementSet
    IGVFDS0238VKNF
    CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
    Charles Gersbach, Duke
    Sample Summary
    Homo sapiens CD8-positive, alpha-beta memory T cell modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
    released
  • principal
    AnalysisSet
    IGVFDS5239SBBT
    activation Perturb-seq: differential gene expression quantifications, filtered feature barcode matrix
    Charles Gersbach, Duke
    Samples
    Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRa Sa-dCas9-VP64, transfected with a guide library
    released