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experimental data
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gRNA sequencing
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guide RNAs
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intermediate analysis
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principal analysis
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samples.@id
/primary-cells/IGVFSM2105CLXS/
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AuxiliarySet
IGVFDS3945FAEL
gRNA sequencing
Charles Gersbach, Duke
Summary
gRNA sequencing for CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
released
intermediate
AnalysisSet
IGVFDS4011VZXE
activation Perturb-seq: filtered feature barcode matrix
Charles Gersbach, Duke
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library
released
MeasurementSet
IGVFDS0238VKNF
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Charles Gersbach, Duke
Sample Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
released
principal
AnalysisSet
IGVFDS5239SBBT
activation Perturb-seq: differential gene expression quantifications
Charles Gersbach, Duke
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library
released
CuratedSet
IGVFDS4729GLXH
guide RNAs
Charles Gersbach, Duke
Summary
Homo sapiens GRCh38 guide RNAs
released