IGVF

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File Set Type

experimental data
1
gRNA sequencing
1
intermediate analysis
1
principal analysis
1

/primary-cells/IGVFSM1190UUKW/

4 items

AuxiliarySet
IGVFDS8372HZNT
gRNA sequencing
Charles Gersbach, Duke
Summary
gRNA sequencing for CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M
released
MeasurementSet
IGVFDS9576VGSP
CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in B2M
Charles Gersbach, Duke
Sample Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of b2m) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
released
principal
AnalysisSet
IGVFDS8839AFQB
CRISPR interference FACS screen targeting B2M: fold change over control, guide quantifications
Charles Gersbach, Duke
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of B2M
released
intermediate
AnalysisSet
IGVFDS2716FVEL
CRISPR interference FACS screen targeting B2M: alignments
Charles Gersbach, Duke
Samples
CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of B2M
released