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File Set Type

4 items
  • AuxiliarySet
    IGVFDS1046ZFUG
    gRNA sequencing
    Charles Gersbach, Duke
    Summary
    gRNA sequencing for CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
    released
  • principal
    AnalysisSet
    IGVFDS5106XTZH
    CRISPR knockout FACS screen targeting IL7R: fold change over control, guide quantifications
    Charles Gersbach, Duke
    Samples
    CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL7R
    released
  • intermediate
    AnalysisSet
    IGVFDS5551EATG
    CRISPR knockout FACS screen targeting IL7R: alignments
    Charles Gersbach, Duke
    Samples
    CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL7R
    released
  • MeasurementSet
    IGVFDS1219SFUJ
    CRISPR knockout FACS screen integrating a guide (sgRNA) library targeting transcription start sites in many genes
    Charles Gersbach, Duke
    Sample Summary
    CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 90-100% expression of IL7R) modified with CRISPRko Sp-Cas9, transduced (lentivirus) with a guide library (MOI of 0.4)
    released