prime editing Variant-EFFECTS targeting IL2RA: variant effects
Jesse Engreitz, Stanford
Samples
Jurkat cell line, stimulated with treatment(s), modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL2RA
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MeasurementSet
IGVFDS6967UVNI
CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Jesse Engreitz, Stanford
Sample Summary
Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library