audit_item_status

audit_analysis_set_files_missing_analysis_step_version

Jesse Engreitz

Stanford Center for Connecting DNA Variants to Function and Phenotype

Analysis of PPIF promoter 8-bp insertion edit effects on PPIF expression that were measured by Variant-FlowFISH in Jurkat cells.

Jesse Engreitz, Stanford

Jurkat cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of PPIF

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 55-65% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 11-20% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-10% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Jurkat cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 90-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 35-45% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 80-90% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Jurkat cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)

prime editing Variant-EFFECTS targeting PPIF: variant effects

audit_inconsistent_sequencing_kit

audit_missing_seqspec

audit_missing_auxiliary_set

audit_targeted_genes

Variant-EFFECTS

guide (sgRNA) library targeting sequence variants in a genomic locus

unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus

Accession

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Assay Term

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Audit category: NOT COMPLIANT

Audit category: WARNING

Audit Category: Internal Action

Externally Hosted

samples.@id

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