Homo sapiens Jurkat cell line, modified with CRISPRa Sa-dCas9-VP64, transfected with a guide library, sorted on expression of IL2RA
released
AuxiliarySet
IGVFDS0128XYUT
gRNA sequencing
Charles Gersbach, Duke
Summary
gRNA sequencing
revoked
MeasurementSet
IGVFDS4959TRIO
CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Charles Gersbach, Duke
Sample Summary
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)