CRISPR prime editing Variant-EFFECTS targeting IL2RA: variant effects
Jesse Engreitz, Stanford
Samples
Homo sapiens Jurkat cell line, stimulated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library, sorted on expression of IL2RA
preview
MeasurementSet
IGVFDS3202JLFH
CRISPR prime editing Variant-EFFECTS sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting sequence variants in a genomic locus
Jesse Engreitz, Stanford
Sample Summary
Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 26-50% expression of IL2RA) treated with 100 ng/mL phorbol 13-acetate 12-myristate for 24 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 24 hours, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library