audit_item_status

audit_inconsistent_sequencing_kit

audit_missing_seqspec

audit_missing_auxiliary_set

audit_targeted_genes

Jesse Engreitz

Stanford Center for Connecting DNA Variants to Function and Phenotype

Jesse Engreitz, Stanford

Variant-EFFECTS

guide (sgRNA) library targeting sequence variants in a genomic locus

Jurkat cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

unsorted FACS input CRISPR prime editing Variant-EFFECTS integrating a guide (sgRNA) library targeting sequence variants in a genomic locus

audit_analysis_set_files_missing_analysis_step_version

Analysis of PPIF promoter Enformer-designed edit effects on PPIF expression that were measured by Variant-FlowFISH in Jurkat cells.

Jurkat cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of PPIF

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Jurkat cell line, male, Homo sapiens (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library

Jurkat cell line, male, Homo sapiens modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)

prime editing Variant-EFFECTS targeting PPIF: variant effects

Accession

Aliases

Alternate Accessions

Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

Award

Description

Donors

Taxa

File Set Type

Functional Assay Mechanisms

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Sample Summary

Samples

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

Status

Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

FileSet

File Set

Sample

Files

Provenance

Quality

Object Type

Classification

Modification

File Type

File Format

Audit category: NOT COMPLIANT

Audit category: WARNING

Audit Category: Internal Action

Externally Hosted

samples.@id

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