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samples.@id
/in-vitro-systems/IGVFSM3148FRBY/
3 items
  • MeasurementSet
    IGVFDS6156TPWX
    CRISPR activation FACS screen sorted on the expression of IL2RA integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
    Charles Gersbach, Duke
    Sample Summary
    Homo sapiens (male) Jurkat cell line (sorting details: FACS bin for 90-100% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
    released
  • AuxiliarySet
    IGVFDS8972XOSG
    gRNA sequencing
    Charles Gersbach, Duke
    Summary
    gRNA sequencing
    revoked
  • principal
    AnalysisSet
    IGVFDS2261TIMI
    CRISPR activation FACS screen targeting IL2RA: alignments, fold change over control, guide quantifications
    Charles Gersbach, Duke
    Samples
    Homo sapiens Jurkat cell line, modified with CRISPRa Sa-dCas9-VP64, transfected with a guide library, sorted on expression of IL2RA
    released