CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
Richard Sherwood, Brigham and Women's Hospital
Samples
Homo sapiens HepG2 cell line, modified with CRISPR base editing SpRY-ABE8e, transfected with a guide library, sorted into bins
released
MeasurementSet
IGVFDS5856FVYJ
CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide
Richard Sherwood, Brigham and Women's Hospital
Sample Summary
Homo sapiens (male) HepG2 cell line (sorting details: Sorted for Top 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)