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samples.@id
/in-vitro-systems/IGVFSM1838CFFU/
4 items
  • AuxiliarySet
    IGVFDS8113ANRK
    gRNA sequencing
    Charles Gersbach, Duke
    Summary
    gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
    released
  • principal
    AnalysisSet
    IGVFDS7196OWBU
    CRISPR activation FACS screen targeting IL2RA: fold change over control, guide quantifications
    Charles Gersbach, Duke
    Samples
    Jurkat cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL2RA
    released
  • intermediate
    AnalysisSet
    IGVFDS5218AVPW
    CRISPR activation FACS screen targeting IL2RA: alignments
    Charles Gersbach, Duke
    Samples
    Jurkat cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL2RA
    released
  • MeasurementSet
    IGVFDS4586UCAI
    CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
    Charles Gersbach, Duke
    Sample Summary
    Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-2xVP64, transduced (lentivirus) with a guide library (MOI of 0.4)
    released