Jurkat cell line, modified with CRISPR modifications, transfected with a guide library, sorted on expression of IL2RA
released
AuxiliarySet
IGVFDS2036IYZB
gRNA sequencing
Charles Gersbach, Duke
Summary
gRNA sequencing for CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
released
MeasurementSet
IGVFDS4954WWBW
CRISPR activation FACS screen integrating a guide (sgRNA) library targeting transcription start sites in IL2RA
Charles Gersbach, Duke
Sample Summary
Jurkat cell line, Homo sapiens (sorting details: FACS bin for 0-10% expression of IL2RA ) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)