IGVF

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Assay

in vitro CRISPR screen using single-cell RNA-seq
4

Preferred Assay Title

scCRISPR screen
4

Auxiliary Data

gRNA sequencing
4

Construct Library Data

guide library
4

Sequencing Platform

Illumina NextSeq 2000
4

/analysis-sets/IGVFDS9078ZWQH/

4 items

MeasurementSet
IGVFDS8535CXVX
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Jay Shendure, UW
Sample Summary
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
released
MeasurementSet
IGVFDS7395DPAD
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Jay Shendure, UW
Sample Summary
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
released
MeasurementSet
IGVFDS4863BMGZ
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Jay Shendure, UW
Sample Summary
K562 cell line (cellular sub pool: 10Xlane1), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library
released
MeasurementSet
IGVFDS0508ERUQ
scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Jay Shendure, UW
Sample Summary
K562 cell line (cellular sub pool: 10Xlane2), female, Homo sapiens modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library
released