IGVF

/

MeasurementSet

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Assay

in vitro CRISPR screen using single-cell RNA-seq
3

Preferred Assay Title

Perturb-seq
3

Auxiliary Data

gRNA sequencing
3

Construct Library Data

guide library
3

Investigated Gene

BACH2
3
BATF
3
BATF3
3
BHLHE40
3
CREM
3
DNMT1
3
EOMES
3
FLI1
3
FOXD2
3
FOXO1
3

Sequencing Platform

Illumina NovaSeq 6000
3

/analysis-sets/IGVFDS3319RFFN/

3 items

MeasurementSet
IGVFDS9674AATW
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Charles Gersbach, Duke
Sample Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
released
MeasurementSet
IGVFDS9813VHFD
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Charles Gersbach, Duke
Sample Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
released
MeasurementSet
IGVFDS1333MBPK
CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Charles Gersbach, Duke
Sample Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)
released