Hon Perturb-seq Workflow

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Gary Hon

Multiscale functional characterization of genomic variation in human developmental disorders

Gary Hon, UT

Homo sapiens GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell, at 12 day(s) post change, modified with CRISPRi Sp-dCas9, transfected with a guide library, cellular sub pool(s): subpool_LW261, subpool_LW262, subpool_LW263, subpool_LW264, subpool_LW265, subpool_LW266, subpool_LW267, subpool_LW268

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW265) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW267) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW268) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW261) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW264) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW262) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW263) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW266) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

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Audit category: NOT COMPLIANT

Audit Category: Internal Action

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