Gersbach FACS CRISPR screen workflow

audit_biosample_age

Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

Homo sapiens CD8-positive, alpha-beta memory T primary cell, modified with CRISPRa Sa-dCas9-VP64, transfected with a guide library, sorted on expression of CCR7

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CCR7) modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

CRISPR activation FACS screen targeting CCR7 integrating a guide (sgRNA) library targeting transcription start sites in many genes

Accession

Aliases

Alternate Accessions

Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

Award

Controlled Access

Data Use Limitation Summaries

Description

Donors

Taxa

File Set Type

File Type

Measured Mechanism

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Simplified Sample Summary

Samples

Sample Institutional Certificates

Sample Summary

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

Status

Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

Object Type

Construct Library Data

Classification

Sample

Modification

File Format

Construct Library Design

Data Use Limitation

Audit category: WARNING

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