Shendure bulk RNA-seq singleton analysis workflow

Jay Shendure

Massively parallel characterization of variants and elements impacting transcriptional regulation in dynamic cellular systems

differential gene expression quantifications

Jay Shendure, UW

Homo sapiens K562 cell line, GM25256 (WTC-11) differentiated cell specimen induced to neuron, at 19 day(s) post change, modified with CRISPRa Sp-dCas9-VP64, CRISPRa Sp-dCas9-VPH, transfected with a guide library

Homo sapiens (female) K562 cell line modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library

CRISPR activation RNA-seq integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

Accession

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Assay Term

Assemblies

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Description

Donors

Taxa

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Measured Mechanism

Input File Sets

Measurement Sets

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Protocols

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Sample Institutional Certificates

Sample Summary

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Selection Criteria

Sequencing Library Types

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Assay Targeted Genes

Transcriptome Annotations

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Construct Library Data

Classification

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Cellular Transformation Target

Modification

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Construct Library Design

Data Use Limitation

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Creation Date

input_file_sets.@id

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