Gersbach FACS CRISPR screen workflow

audit_biosample_age

Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

Homo sapiens CD8-positive, alpha-beta memory T primary cell primary cell, modified with CRISPRi Sa-dCas9-KOX1-KRAB, transfected with a guide library, sorted on expression of CD2

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CD2 ) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 0-10% expression of CD2) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

Homo sapiens CD8-positive, alpha-beta memory T cell (sorting details: FACS bin for 90-100% expression of CD2) modified with CRISPRi Sa-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library (MOI of 0.4)

CRISPR interference FACS screen targeting CD2 integrating a guide (sgRNA) library targeting transcription start sites in CD2: alignments, fold change over control, guide quantifications

Accession

Aliases

Alternate Accessions

Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

Award

Description

Donors

Taxa

File Set Type

Measured Mechanism

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Simplified Sample Summary

Samples

Sample Institutional Certificates

Sample Summary

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

Status

Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

Object Type

Construct Library Data

Classification

Sample

Modification

File Type

File Format

Construct Library Design

Data Use Limitation

Audit category: WARNING

input_file_sets.@id

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