MAGeCK CRISPR screen workflow

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Luca Pinello

Comprehensive characterization of variants underlying heart and blood diseases with CRISPR base editing

Guillaume Lettre, MHI

Homo sapiens TeloHAEC cell line, modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Homo sapiens (female) TeloHAEC cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB, transduced (lentivirus) with a guide library

Proliferation CRISPR interference screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide associated with blood pressure: guide quantifications

Accession

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Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

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Description

Donors

Taxa

File Set Type

Measured Mechanism

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Simplified Sample Summary

Samples

Sample Institutional Certificates

Sample Summary

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

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Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

Object Type

Construct Library Data

Classification

Sample

Modification

File Type

File Format

Construct Library Design

Data Use Limitation

Audit Category: Internal Action

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