BEAN CRISPR screen workflow

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Luca Pinello

Comprehensive characterization of variants underlying heart and blood diseases with CRISPR base editing

Richard Sherwood, Brigham and Women's Hospital

Homo sapiens HepG2 cell line, modified with CRISPR base editing SpRY-ABE8e, transfected with a guide library, sorted into bins

Homo sapiens (male) HepG2 cell line (sorting details: Sorted for Top 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

Homo sapiens (male) HepG2 cell line (sorting details: Sorted for Bottom 20% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

Homo sapiens (male) HepG2 cell line (sorting details: Sorted for Bottom 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

Homo sapiens (male) HepG2 cell line (sorting details: Sorted for Top 20-40% LDL-C uptake) modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

Homo sapiens (male) HepG2 cell line modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library (MOI of 0.5)

CRISPR base editing FACS screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide

Accession

Aliases

Alternate Accessions

Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

Award

Controlled Access

Data Use Limitation Summaries

Description

Donors

Taxa

File Set Type

File Type

Measured Mechanism

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Simplified Sample Summary

Samples

Sample Institutional Certificates

Sample Summary

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

Status

Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

Object Type

Construct Library Data

Classification

Sample

Modification

File Format

Construct Library Design

Data Use Limitation

Audit Category: Internal Action

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