Variant-EFFECTS Pipeline

audit_item_status

audit_sample_missing_publication

audit_file_set_missing_publication

Jesse Engreitz

Stanford Center for Connecting DNA Variants to Function and Phenotype

Analysis of PPIF promoter Enformer-designed edit effects on PPIF expression that were measured by Variant-FlowFISH in THP-1 cells.

Jesse Engreitz, Stanford

Homo sapiens THP-1 cell line, modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transfected with a guide library, sorted on expression of PPIF

Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 76-100% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 26-50% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 51-75% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Homo sapiens (male) THP-1 cell line (sorting details: FACS bin for 0-25% expression of PPIF) modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Homo sapiens (male) THP-1 cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2), transduced (lentivirus) with a guide library

Homo sapiens (male) THP-1 cell line modified with CRISPR prime editing Sp-nCas9-M-MLV RT (PE2)

CRISPR prime editing Variant-EFFECTS targeting PPIF: variant effects

Accession

Aliases

Alternate Accessions

Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

Award

Description

Donors

Taxa

File Set Type

Measured Mechanism

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Simplified Sample Summary

Samples

Sample Institutional Certificates

Sample Summary

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

Status

Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

Object Type

Construct Library Data

Classification

Sample

Modification

File Type

File Format

Data Use Limitation

Audit Category: Internal Action

input_file_sets.@id

Search