Satpathy Cell Ranger

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Jesse Engreitz

Stanford Center for Connecting DNA Variants to Function and Phenotype

Quality-control workflow performed by Satpathy Lab - K562-CRISPRi, THP1, Jurkat, Jurkat + CD3/PMA, TeloHAEC-CRISPRi

Ansuman Satpathy, Stanford

K562, THP-1, Jurkat, TeloHAEC cell line and multiplexed sample, stimulated with treatment(s), modified with CRISPR modifications

multiplexed sample: Jurkat cell line, male, Homo sapiens treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours; K562 cell line, female, Homo sapiens modified with CRISPRi Sp-dCas9-KOX1-KRAB; ... and 8 more samples

Treatment of 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours

Treatment of 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours

10x multiome with MULTI-seq: cell hashing barcodes, fragments, index, peaks, raw feature barcode matrix

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Audit Category: Internal Action

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