MAGeCK CRISPR screen workflow

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Luca Pinello

Comprehensive characterization of variants underlying heart and blood diseases with CRISPR base editing

Daniel Bauer, HSCI

Homo sapiens HUDEP-2 cell line, modified with CRISPR base editing SpRY-ABE8e, CRISPR cutting SpRY, transfected with a guide library

Homo sapiens HUDEP-2 cell line modified with CRISPR cutting SpRY, transduced (lentivirus) with a guide library

Homo sapiens HUDEP-2 cell line modified with CRISPR base editing SpRY-ABE8e, transduced (lentivirus) with a guide library

Proliferation CRISPR base editing, cutting screen integrating a guide (sgRNA) library targeting phenotype-associated variants genome-wide

Accession

Aliases

Alternate Accessions

Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

Award

Controlled Access

Data Use Limitation Summaries

Description

Donors

Taxa

File Set Type

File Type

Measured Mechanism

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Simplified Sample Summary

Samples

Sample Institutional Certificates

Sample Summary

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

Status

Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

Object Type

Construct Library Data

Classification

Sample

Modification

File Format

Construct Library Design

Data Use Limitation

Audit Category: Internal Action

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