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Jesse Engreitz

Stanford Center for Connecting DNA Variants to Function and Phenotype

Analysis of TLNRD1 CRIPSRi enhancer screen with FlowFISH in TeloHAEC

Jesse Engreitz, Stanford

Homo sapiens TeloHAEC cell line, modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library, sorted on expression of TLNRD1

Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 26-50% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 76-100% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 51-75% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

Homo sapiens (female) TeloHAEC cell line (sorting details: FACS bin for 0-25% expression of TLNRD1) modified with CRISPRi Sp-dCas9-KOX1-KRAB, transfected with a guide library

CRISPR interference FlowFISH screen targeting TLNRD1 integrating a guide (sgRNA) library targeting candidate cis-regulatory elements in a genomic locus

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Audit category: ERROR

Audit category: NOT COMPLIANT

Audit category: WARNING

Audit Category: Internal Action

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