Satpathy Cell Ranger

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Ansuman Satpathy

Single-cell Mapping Center for Human Regulatory Elements and Gene Activity

Quality-control workflow performed by Satpathy Lab - K562-CRISPRi, THP1, Jurkat, Jurkat + CD3/PMA, TeloHAEC-CRISPRi

Ansuman Satpathy, Stanford

Homo sapiens multiplexed sample of Jurkat, K562, THP-1, TeloHAEC, stimulated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours, modified with CRISPRi Sp-dCas9-KOX1-KRAB

multiplexed sample: Homo sapiens (male) Jurkat cell line treated with 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours, 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours; Homo sapiens (female) K562 cell line modified with CRISPRi Sp-dCas9-KOX1-KRAB; ... and 8 more samples

Treatment of 100 ng/mL phorbol 13-acetate 12-myristate for 4 hours

Treatment of 5 μg/mL T-cell surface glycoprotein CD3 gamma chain for 4 hours

single-nucleus ATAC-seq (10x multiome with MULTI-seq), single-nucleus RNA sequencing assay (10x multiome with MULTI-seq)

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Audit Category: Internal Action

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