Hon Perturb-seq Workflow

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Gary Hon

Multiscale functional characterization of genomic variation in human developmental disorders

unfiltered global differential expression

Gary Hon, UT

Homo sapiens GM25256 (WTC-11) cell line, modified with CRISPRi Sp-dCas9, transfected with a guide library

Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW288) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW289) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW287) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

Homo sapiens (male) GM25256 (WTC-11) cell line (cellular sub pool: subpool_LW286) modified with CRISPRi Sp-dCas9, transduced (lentivirus) with a guide library

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Accession

Aliases

Alternate Accessions

Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

Award

Controlled Access

Data Use Limitation Summaries

Description

Donors

Taxa

File Set Type

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Measured Mechanism

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Simplified Sample Summary

Samples

Sample Institutional Certificates

Sample Summary

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

Status

Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

Object Type

Construct Library Data

Classification

Sample

Modification

File Format

Construct Library Design

Data Use Limitation

Audit category: NOT COMPLIANT

Audit Category: Internal Action

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