Hon Perturb-seq Workflow

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Gary Hon

Multiscale functional characterization of genomic variation in human developmental disorders

unfiltered global differential expression

Gary Hon, UT

Homo sapiens H9 differentiated cell specimen induced to cardiac muscle cell, at 12 day(s) post change, modified with CRISPRi Sp-dCas9, transfected with a guide library

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW431) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW433) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW434) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

Homo sapiens (female) H9 differentiated cell specimen induced to cardiac muscle cell for 12 days (cellular sub pool: subpool_LW432) modified with CRISPRi Sp-dCas9, 3 week(s) after transfection with a guide library

CRISPR interference Perturb-seq integrating a guide (sgRNA) library targeting accessible genome regions in many genomic loci

Accession

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Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

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Data Use Limitation Summaries

Description

Donors

Taxa

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Measured Mechanism

Input File Sets

Measurement Sets

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Prediction Objects

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Protocols

Simplified Sample Summary

Samples

Sample Institutional Certificates

Sample Summary

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Sequencing Library Types

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Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

Object Type

Construct Library Data

Classification

Sample

Cellular Transformation Target

Modification

File Format

Construct Library Design

Data Use Limitation

Audit category: NOT COMPLIANT

Audit Category: Internal Action

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