Shendure scCRISPRa Pipeline

Cell Ranger Gene Count Pipeline

audit_file_no_file_format_specifications

audit_missing_transcriptome

Jay Shendure

Massively parallel characterization of variants and elements impacting transcriptional regulation in dynamic cellular systems

differential gene expression quantifications

Jay Shendure, UW

Homo sapiens K562 cell line, modified with CRISPRa Sp-dCas9-VP64, CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library, cellular sub pool(s): 10Xlane1, 10Xlane2

Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane2) modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane2) modified with CRISPRa Sp-dCas9-VP64, transfected with a guide library

Homo sapiens (female) K562 cell line (cellular sub pool: 10Xlane1) modified with CRISPRa Sp-dCas9-VP64-p65-Rta (VPR), transfected with a guide library

scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci

Accession

Aliases

Alternate Accessions

Assay Term

Assemblies

Assessed Genes

Associated Phenotypes Name

Award

Controlled Access

Data Use Limitation Summaries

Description

Donors

Taxa

File Set Type

File Type

Measured Mechanism

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Simplified Sample Summary

Samples

Sample Institutional Certificates

Sample Summary

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

Status

Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

Object Type

Construct Library Data

Classification

Sample

Modification

File Format

Construct Library Design

Data Use Limitation

Release Date

Creation Date

Audit category: NOT COMPLIANT

input_file_sets.@id

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