Gersbach Perturb-seq workflow

audit_biosample_age

Charles Gersbach

High-Throughput Functional Annotation of Gene Regulatory Elements and Variants Critical to Complex Cellular Phenotypes

Charles Gersbach, Duke

CD8-positive, alpha-beta memory T cell primary cell, modified with CRISPR modifications, transfected with a guide library

CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)

activation Perturb-seq: differential gene expression quantifications

Accession

Aliases

Alternate Accessions

Assay Term

Assemblies

Assessed Genes

Associated Phenotypes

Award

Description

Donors

Taxa

File Set Type

Functional Assay Mechanisms

Input File Sets

Measurement Sets

Model Name

Prediction Objects

Preferred Assay Title

Protocols

Sample Summary

Samples

Prediction Scope

Selection Criteria

Sequencing Library Types

Genes

Status

Summary

Assay Targeted Genes

Transcriptome Annotations

UUID

FileSet

File Set

Sample

Files

Provenance

Quality

Object Type

Classification

Modification

File Type

File Format

Audit category: WARNING

input_file_sets.@id

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