CRISPR interference FACS screen integrating a guide (sgRNA) library targeting transcription start sites in CD2
Charles Gersbach, Duke
Sample Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens (sorting details: FACS bin for 0-10% expression of CD2 ) modified with CRISPRi Sa-dCas9, transduced (lentivirus) with a guide library (MOI of 0.4)