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MeasurementSet
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Assay
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in vitro CRISPR screen using single-cell RNA-seq
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Preferred Assay Title
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Perturb-seq
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Auxiliary Data
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gRNA sequencing
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Construct Library Data
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guide library
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Investigated Gene
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BACH2
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BATF
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BATF3
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BHLHE40
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CREM
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DNMT1
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EOMES
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FLI1
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FOXD2
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FOXO1
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Sequencing Platform
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Illumina NovaSeq 6000
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auxiliary_sets.@id
/auxiliary-sets/IGVFDS9108NLTS/
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MeasurementSet
IGVFDS6764PIJI
CRISPR activation Perturb-seq integrating a guide (sgRNA) library targeting transcription start sites in 24 genes
Charles Gersbach, Duke
Sample Summary
CD8-positive, alpha-beta memory T cell, Homo sapiens modified with CRISPRa Sa-dCas9-VP64, transduced (lentivirus) with a guide library (MOI of 0.4)
released