scCRISPR activation screen integrating a guide (sgRNA) library targeting TF binding sites, candidate cis-regulatory elements in many genomic loci
Jay Shendure, UW
Sample Summary
Homo sapiens (male) GM25256 (WTC-11) differentiated cell specimen induced to neuron for 19 days (cellular sub pool: 10Xlane4) modified with CRISPRa Sp-dCas9-VPH, transfected with a guide library